Mops 1x buffer

Common Reagents. BioTechniques Molecular Biology Techniques Forums • View topic. Denaturing Agarose GE. doc.

RNA Guide: Working with RNA. Northern Blots.

Agarose Gel for Southerns(DNA): 0.8% - 1.2% in 1X TBE Buffer Preparation Agarose Gel for Northerns(RNA): 1.2% in 1X MOPS Buffer, 0.67M Formaldehyde. 12 Jan 2004 Add 5 ml of 10x MOPS-EDTA buffer and 43.25 ml DEPC treated H2O. 3. Make up a 1x MOPS-EDTA buffer, and pour into gel chamber. 13.

Mini SDS-PAGE Gel Protocol for the Novex Surelock Cell

Prior to running the gel, equilibrate in 1x FA gel running buffer (see buffer ·200 mM 3-[N-morpholino]propanesulfonic acid (MOPS) (free acid). 10X MOPS Buffer (200 mm MOPS, 10 mM EDTA, 50 mM NaAcetate, pH 7.0 KOH) 37% (v/v) formaldehyde. 1X MOPS Buffer RNA Sample Buffer (20 mM MOPS.

Is there a difference if MOPS or MOPSO used as buffers in

SERVA Tris-MOPS/SDS - Instruction Manual - Biophoretics. Denaturing formaldehyde agarose gel for RNA - General Lab. 1X MOPS running buffer (30ml 10x MOPS buffer made above & 270 ml DEPC. d H2O) (Note: the buffer ends up about pH 5, is that a problem).

Running formaldehyde gels in 1x MOPS buffer - Google Groups. Northern Blotting - Molecular Neurogenetics.

Northern Blot.

Reagent preparation

Buffer A. 294µL 10X MOPS/EDTA. 106µL RNase-free water. Gel Loading Buffer Prepare a 1% agarose gel in 1X MOPS/EDTA buffer and cool to 600C. 3. To prepare 1 l 1x running buffer: To 50 ml 20x concentrate add 950 ml deionized water. Concentration of components in 1x solution: 0.06 M TRIS. 0.03 M MOPS. Dissolve 41.8g of MOPS, 6.8g of Sodium Acetate, 3.8g of Disodium EDTA in at 5-75V/cm for 2 hours in 250 ml 1X MOPS or 1 X Ambion Gel Running Buffer.